Microbiology Spring 2007

medhead · Mar 6, 2007

So, if someone wants to throw out some topics to me, I'd forever be in your debt of thankfullness. :)
Jenny

I'd do a paper on E. coli...specifically 0157:H7. My brother came very close to dying from that particular strain a few years ago.

LisaJ_RN · Mar 6, 2007

how about something about Micobacterium smegmatis :) (to go with the heated circumcision thread) :monkeydance:

catzy5 · Mar 7, 2007

here is a micro question for you guys. I am doing my lab assignment and I am stumped..

"How might fluorescence microscopy be used to visualize the bacterial capsule?"

Its not in the lab packet and I read about fluoroescence microscopy and I know what a capsule is but I can't see how it would be used to identify one.

anyone?

BlessedMom · Mar 9, 2007

I am not totally sure but I do know that when you use fluorsence microscopy with capsulated bacteria it doesn't stain it but a fluorsent halo is around them.

biogeek · Mar 9, 2007

Fluorochrome dyes will tend to illuminate your specimen under UV light under normal fluorecence microscopy. You can also add a fluorescent dye to an antibody that is specific for that microorganism & then detect the microorganism. If the antibody doesn't have an antigen to adhere to on the microorganism, it'll get washed away & you won't see a darn thing under the scope. You can use this technique to diagnose syphillis for example. However, this is immunofluorescence, a specific subset of the original.

Not all certain how in-depth your professor wants to go. If you want more in-depth info, just let me know.

catzy5 · Mar 9, 2007

I am not totally sure but I do know that when you use fluorsence microscopy with capsulated bacteria it doesn't stain it but a fluorsent halo is around them.

Thanks that sounds like a good answer to me!

GeneralJinjur · Mar 9, 2007

And I survived. It was much easier than the A&P lab practicals and it didn't smell icky, either. We weren't timed at the stations, so we could move around freely. I was feeling very confident and headed straight to the dissecting scope at the beginning, then drew a blank on Fasciola hepatica and slid to the next station, which I did recognize. I never had time to make my organism wallpaper, but I did get them all memorized by domain, kingdom and genus. Afterward, we had to stay and do a full lab, but now I get a week off to clean my house and read ahead! Maybe I'll go to bed at a decent hour, too. I am such a Spring Break party animal!

Denise

catzy5 · Mar 10, 2007

And I survived. It was much easier than the A&P lab practicals and it didn't smell icky, either. We weren't timed at the stations, so we could move around freely. I was feeling very confident and headed straight to the dissecting scope at the beginning, then drew a blank on Fasciola hepatica and slid to the next station, which I did recognize. I never had time to make my organism wallpaper, but I did get them all memorized by domain, kingdom and genus. Afterward, we had to stay and do a full lab, but now I get a week off to clean my house and read ahead! Maybe I'll go to bed at a decent hour, too. I am such a Spring Break party animal!
Denise

congrats! we just had our practical last week too I had micro on thursday and AP on Saturday I got a 97 on both. The micro was funny she did it all on power point, Ap was the traditional walk through. We have another month before spring break I can't wait! I will do the samething as you lol.

cesRN · Mar 10, 2007

Spring Break! Finally! Not really a break since we have a lab practical and Unit II as soon as we get back. I am holding on to a 93 average and I'm planning on keeping that way or increasing it with extra credit. Good luck to everybody! And have fun along with the studying! That is the only way you will be satisfied with your results :-)

p.s. Group study helps!

np_wannabe · Mar 10, 2007

congrats! we just had our practical last week too I had micro on thursday and AP on Saturday I got a 97 on both. The micro was funny she did it all on power point, Ap was the traditional walk through. We have another month before spring break I can't wait! I will do the samething as you lol.

So funny....being a 30-something....I was just thinking tonight that once my lab practical is over (tomorrow), I only have two weeks left until spring break and then I can clean MY house and get to sleep before midnight!! If only my friends of 15 years ago could see me now....they wouldn't recognize me!! :lol2:

qaqueen · Mar 10, 2007

Hey Jenny,

How about something newsy like genetic changes in EIDs as they move into the US

BlessedMom · Mar 10, 2007

So my spring break has arrived. I am sitting here thinking about studying some more of my AP2 whilst I wait. I just cleaned my house and now I am feeling guilty about not studying. How sad is that? lol. Halfway through and I have a 98% in Micro so I am pretty excited and hoping I can keep it that high! Our next bits are on the actual diseases! I am so excited. Last class we looked at a bunch of conditions. It was pretty amazing.