ø¤º°* Medical Microbiology Winter 2006 Club*°º¤ø

[kayel]

okay, people! who is with me on this one? i am feeling a little nervous about all those slides! i'm looking forward to meeting everyone in this course!

i will gather up any useful sites i find in the last med micro club and elsewhere and put them in this post!

here's a start ..

some sites from fall 05 club:

• microbes in sickness and in health (niaid)
  http://www.niaid.nih.gov/publications/microbes.htm
  
• microbelibrary (american society for microbiology)
  http://www.microbelibrary.org/index.asp
  
• microbes.info
  http://www.microbes.info/index.html
  
• "the grapes of staph" -- doc kaiser's microbiology website http://student.ccbcmd.edu/~gkaiser/goshp.html
  
• "cells alive"
  http://www.cellsalive.com/
  
• parasites and parasitological resources
  http://www.biosci.ohio-state.edu/~parasite/home.html
  
• fun2care's micro quizzes
  http://www.nursingjourney.com/quizzes.html
  
• micro e-guide
  http://www.microeguide.com/toc_safe_frame.htm
  
• university of hawaii microbiology faculty site
  http://www2.hawaii.edu/~johnb/micro/index.html
  
• photo gallery of bacterial pathogens
  http://www.geocities.com/capecanaveral/3504/gallery.htm
  
• nikon microscopyu
  http://www.microscopyu.com/sitemap.html
  
• the gram stain
  http://www-micro.msb.le.ac.uk/video/gram.html
  

• biol 230 microbiology lecture guide unit 1

  
  http://www.cat.cc.md.us/courses/bio141/lecguide/unit1/index.html
  

• indoor fungus resources
  http://www.dehs.umn.edu/iaq/fungus/pictures.html
  
• microbiology articles
  http://bio.classes.ucsc.edu/bio119/winter04/webpgs_papers.htm
  
• richland college, lab procedures manual
  http://www.rlc.dcccd.edu/mathsci/reynolds/micro/lab_manual/toc.html

[stpauligirl]

My micro teacher gave us our grades so far in the class. I have a 91%. It's an A, but just barely. I feel like I can lose it at any time.

You can do it, the class is half way over....keep holding on

I am doing very well and I am at more than 100% after 3 lecture tests, my lab is somewhere in the mid 90's and will go up after my unknown presentation and the completion of all lab quizzes. I hope everyone will have a good Spring Break!

[jenmomto3]

Hello,

I just found this site and I am so HAPPY!! I am such a puter geek and love message boards.. Let me into myself. I am a mother of three children (under the age of five) ( yes, I am barely sain) lol I am taking five classes this semester and micro is one of them. I just got my acceptance letter for the nursing program that starts in the fall. YEAH!!!!! i cant wait to meet all of you.. good luck in your microbe endevors....

Jenny

[stpauligirl]

Hello,

I just found this site and I am so HAPPY!! I am such a puter geek and love message boards.. Let me into myself. I am a mother of three children (under the age of five) ( yes, I am barely sain) lol I am taking five classes this semester and micro is one of them. I just got my acceptance letter for the nursing program that starts in the fall. YEAH!!!!! i cant wait to meet all of you.. good luck in your microbe endevors....

Jenny

Nice to meet you and good luck

[Elizabeth Hanes, BSN, RN]

Our second micro test was Weds, and I think I did quite well. Won't have the grades for TWO WEEKS (gasp!) due to Spring Break.

Still, doing the happy dance. Hope everyone else is still hanging in there.

[Staric]

HI- Does anyone have a good website to learn serial dilution's? My book does not explain it well enough for me to wrap my head around a real question: You have 500,000 cells to start and you do a 10 (little 4) dilution in a step series of 1:10 dilutions, how many cells do you have? None of my answers match the choices...:

[stpauligirl]

HI- Does anyone have a good website to learn serial dilution's? My book does not explain it well enough for me to wrap my head around a real question: You have 500,000 cells to start and you do a 10 (little 4) dilution in a step series of 1:10 dilutions, how many cells do you have? None of my answers match the choices...:

We haven't gotten to that part in our course yet but here is a formula that I found:

Number of colonies on plate X reciprocal of dilution of sample = number of bacteria/ml

For example: if 32 colonies are on a plate of 1/10,000 dilution, then the count is 32 X 10,000 = 320,000/ml in sample

[stpauligirl]

HI- Does anyone have a good website to learn serial dilution's? My book does not explain it well enough for me to wrap my head around a real question: You have 500,000 cells to start and you do a 10 (little 4) dilution in a step series of 1:10 dilutions, how many cells do you have? None of my answers match the choices...:

I think you do it this way:

500,000 divided by 1:10 = 50,000 cells after the first dilution (first tube)

500,000 divided by 1:100 = 5000 cells after the second dilution (second tube)

500,000 divided by 1:1000 = 500 cells after the third dilution (third tube)

500,000 divided by 1;10,000 = 50 celss after the fourth dilution (fourth tube)

500,000 divided by 1:100,000 = 5 cells after the fifth dilution (fifth tube)

In serial dilutions, the original inoculum is diluted in a series of dilution tubes. Each succeeding dilllution tube will have only on-tenth the number of microbial cells as the preceding tube.

[Staric]

That totally makes sense!!!!!!!!!!!!!!!!! Thank YOU!!!!

[stpauligirl]

That totally makes sense!!!!!!!!!!!!!!!!! Thank YOU!!!!

I am so glad that I was able to help....I wasn't sure if I wrote it in a way so it would make sense. Good luck with it !

[Elizabeth Hanes, BSN, RN]

I hope someone can help me.

For our unknowns in lab, we have to write a lab report. I have no idea how to do this. I last took a biology class/lab about 25 years ago, and I don't remember ever having to write a lab report. Our lab TA gave us a flow chart to use in figuring out what our unknown is, but he didn't say a word about how to write a lab report. I think this is one of those things we're "supposed" to know from our pre-requisite classes.

Does anyone know of a good link to a sample lab report? Or can you give me some pointers on what I'm supposed to cover and how to format one?

I much appreciate any help!!

[stpauligirl]

I hope someone can help me.

For our unknowns in lab, we have to write a lab report. I have no idea how to do this. I last took a biology class/lab about 25 years ago, and I don't remember ever having to write a lab report. Our lab TA gave us a flow chart to use in figuring out what our unknown is, but he didn't say a word about how to write a lab report. I think this is one of those things we're "supposed" to know from our pre-requisite classes.

Does anyone know of a good link to a sample lab report? Or can you give me some pointers on what I'm supposed to cover and how to format one?

I much appreciate any help!!

We have to document all the biochemical tests that we perform on our unknown, date and results....I am wondering if you have recorded those and you could include those in your lab report. Also we will be interviewed about these tests (oral presentation of the unknown) and need to be able to answer questions about the uniqueness of these tests and how they came out for our specific unknown.... did you do a streak plate and record observations about your unknowns growth pattern, color etc. We have to report those findings, as well as growth on our initial slant. And don't forget your stains.....what are the results of your Gram..... - or +, what shape your specimen is (basillus or cocci) also report if you have an acid fast organism, if it is spore producing etc.

I hope this helped some and feel free to ask further questions.

[Kathyz]

I'm still having difficulty with the whole Selective and Differential thing. Can anyone help out? Why is Mannitol Salt Agar both selective and differential? Any helpful sites? Thanks!

:smackingf