Microbiology study group spring 2009

afranklin · Feb 2, 2009

CONGRATULATIONS!!

Ms. Nurse Assistant, LPN · Feb 2, 2009

what are the similarities between molecules, atoms and compounds?

Pandorajinel · Feb 2, 2009

molecules are made of two or more elements (atoms). Compounds are made of two or more totally different atoms. So H would be an atom, H2 a molecule, H2O a compound.

tfleuter, BSN, RN · Feb 2, 2009

Here's a lab-related question!

When using a gram stain, what would happen if you reversed the crystal violet and safranin stains? (applied the pink first, and then the purple/blue)

phoenixRisin9 · Feb 2, 2009

Here's a lab-related question!
When using a gram stain, what would happen if you reversed the crystal violet and safranin stains? (applied the pink first, and then the purple/blue)

Hey tfleuter, interesting question. I have the gram-staining lab this afternoon, so I'll see what happens!

cara922 · Feb 2, 2009

tlfleuter, someone asked the prof this in lab. Your gram positive would then be red, and your gram neg would be purple. However she said it is never done it this order. she said it has always been the violet first, and then safranin and that is the way it will always be done in a lab.

NewGoalRN · Feb 3, 2009

I had my first Micro test today and it was a doozer. 100 questions in 1hr 15mins. It was formated like the NCLEX per the professor. I studied, and studied for this test. It will be intersting to see how I did. Now on to the Chemistry of the cell i.e Chemistry...uggghhh!

phoenixRisin9 · Feb 3, 2009

Hey tfleuter, although we did gram-staining lab this afternoon I didn't have time to test using the safranin stain first. When I asked the professor, he said the same as cara922 commented. He didn't elaborate but said that there would likely be mistakes--whatever he meant by that. I don't see why it would make a difference as they are both used as basic stains other than the standard protocol being that you always use crystal violet as the primary stain.

bluemalibu · Feb 3, 2009

In gram negative bacteria, it is actually the color of stain that follows the acetone bath that will apply the subsequent coloring to the microbe. The acetone gives a permeability to the protective shell of the microbes, allowing the stain to reach the peptidogliclan and gain a foothold.

~Blue

Ms. Nurse Assistant, LPN · Feb 3, 2009

how many electrons can fit into the first and second energy levels of the following

carbon

hydrogen

nitrogen

oxygen

what are the similarities and differences between monomers and polymers?

Ms. Nurse Assistant, LPN · Feb 3, 2009

list 3 connections between amino acids, polymers and proteins

Pandorajinel · Feb 3, 2009

how many electrons can fit into the first and second energy levels of the following
carbon
hydrogen
nitrogen
oxygen
what are the similarities and differences between monomers and polymers?

Electron shells are constant. 2 in the first 8 in all others. So fill the first shell with two electrons, the remaining shell.

Carbon: Can accept 4 more in its second level

Hydrogen: can accept 1 more in its first level

Nitrogen: Can accept 3 in its second level

Oxygen: can aceept 2 into its second level