Microbiology Spring 2007 - page 21

:welcome: Gonna try to stick this one!!! Heres to a wonderful semester for all us MBers :):welcome:... Read More

  1. by   Ebony06
    Just remember this: the property of gram-positive bacteria bacillus (rods) is that they form endospores. All gram-positive bacillus (rods) are endospore-formers. Here is the website that helped me through microbiology.

    http://en.wikipedia.org/wiki/Bacillus
    http://microbewiki.kenyon.edu/index.php/MicrobeWiki
  2. by   catzy5
    I am excited it was my first day back after spring break and we are going to start our unknowns now, we did a practice today I got frustrated at first mine seemed to be a lot less straightforward then others, but I did some research and I think I found out my practice unknown. I can't wait for the real thing now its going to be fun.
  3. by   JaxiaKiley
    I just signed up for my summer micro class. I'm so nervous to try to cram this class into a short summer session!
  4. by   JDLAStew
    We started our unknowns tonight. The first agar plate I inoculated, I stabbed!!! I was freaking out! After doing the Gram stain, I'm almost 100% positive I have E. coli. I had some large, very pink rods.
  5. by   ally21
    JDLAStew, that's great you've sorta figured out what your unknown is. Did you have to do other tests to come up with a conclusion? My professor says colony characteristics and gram staining are subjective so we had to do more tests and come up with a reason why we did them. We weren't allowed to streak EMB or Mannitol agars since she said it'd be too easy to narrow down the list. Nice teacher, huh? We're just finishing up ours and I have yet to write my paper. I have until April 16th... Micro has been so stressful and I don't know whether or not to study for the exam or work on the unknown. My professor loves to knock as much points off of us as possible. I pray that I'll pass it this semester so I won't have to ever take it again. It's my last pre-req for the nursing program thank god. Now I'll just have to worry about the nursing classes each semester which shouldn't be so bad.
  6. by   catzy5
    Quote from ally21
    JDLAStew, that's great you've sorta figured out what your unknown is. Did you have to do other tests to come up with a conclusion? My professor says colony characteristics and gram staining are subjective so we had to do more tests and come up with a reason why we did them. We weren't allowed to streak EMB or Mannitol agars since she said it'd be too easy to narrow down the list. Nice teacher, huh? We're just finishing up ours and I have yet to write my paper. I have until April 16th... Micro has been so stressful and I don't know whether or not to study for the exam or work on the unknown. My professor loves to knock as much points off of us as possible. I pray that I'll pass it this semester so I won't have to ever take it again. It's my last pre-req for the nursing program thank god. Now I'll just have to worry about the nursing classes each semester which shouldn't be so bad.

    I hear ya I am not so much worried about doing the unknown as I am about writing the paper! mine isn't due until May but we have started workig on it and started writing the papers she is very particular about the whole thing.
  7. by   akspudus
    We finished up our unknowns last week. First week was setting up all the cultures, second week was reading results.
    I spent the week in between looking up all the possible test results for each of the bacteria possibilities. (which is a lot harder than it seams)
    I think I nailed it all correctly, but I don't find out for sure until tomorrow -> after the written final.

    Akspudus
  8. by   JDLAStew
    I know I'm probably jumping the gun--we just started last night and we plated in agar at 37C and room temp and did one Gram stain. I'll get a little more clarity with each test. We're lucky cause we do get to use EMB, MSA, and MA to determine. I'm with the rest of you--the paper terrifies me! The paper/unknown is 50% of our grade!
  9. by   prinsessa
    We aren't doing unknowns since the equipment at school isn't working right now. We only had a couple of experiments. I was kind of looking forward to lab since I find this stuff interesting. Now we have to listen to the teacher lecture for hours every night.
  10. by   GeneralJinjur
    That's too bad about the equipment. I wouldn't mind more lecture time, but it would be exhausting to go for 3 hours every night.

    Our school does unknowns differently. We cultured whatever we wanted at the beginning of February and have performed every test on known organisms and our unknowns throughout the semester. My writeup is due Tuesday. It's only a tenth of the total grade, but 100 points is a whole grade point.
  11. by   akspudus
    We had a lab journal that we had to maintain through the semester -> 20% of your grade.
    Then we had our fist unknown. More like a mid term exercise-> 20% of your grade
    Our Second unknown finished up last week and this week we had our final practicum -> combined for 35% of your grade.
    The rest of our grade came from quizzes and homework.

    Found out today that I pulled a 93% overall --> Phew...glad it is all over. I hate classes that throw so much weight into one or two exercise. Feels like you have all your eggs in one basket. Oh well, I guess I should get used to it for NS.

    Akspudus
    Miami Dade College
    Barry University Hopeful
  12. by   JDLAStew
    I jumped the gun. And I'm stuck and ready to cry...hard. Tonight my gram stain looked pink and some were purple! All I know is that it's a definite rod. It's between B.cereus, B.Subtilis, E.Coli, K.Pneumoniae, and P. Vulgaris. Those are the only rods we grew this year. My agar was creamy white and shiny but it went crazy! Almost my whole plate was full and I did a simple streak. I'm scared to death. If the whole class wasn't in there tonight, I swear I could have cried. I felt that huge lump in my throat forming. I was way too confident before. I don't see how the Gram stain could have gone from such a brilliant pink to almost a light purple/dk. purple. A gram stain of subtilis on the net looked like mine did tonight. I'm scared to death!!!
    Last edit by JDLAStew on Apr 13, '07 : Reason: Just read this again. Boy was it late--my grammar was BAD! LOL
  13. by   ally21
    JDLAStew, relax! Did you make a back up slant of your unknown? It's possible that there was contamination or the gram stain wasn't right. Did the teacher check the first time you did a gram stain to see if you were on the right track? What other tests have you done? I don't have my chart down here from the Bergey's manual so I'm not much help right now... but I think something may be going wrong for you to have gram + and gram - rods in your unknown.

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