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Hi everyone! I need your help IDing my unknown.
I ran some tests and here are the results:
1. Starch Hydrolysis: positive
2. OF Glucose: negative
3. Fermentation Tube: Glucose: positive, gas negative. Lactose: negative, gas negative. Sucrose: positive, gas negative
4. Citrate: negative
5. Urease: positive, slow
6. Phenylalanine: negative
7. Hydrogen sulfide: negative
8. Motility: negative
9. Ornithine: negative
10. Indole: negative
11. Oxidase: negative. (Although I'm not that sure cause it only turned blue. not pink to blue to black.)
12. Catalase: posiive
13. MRVP: negative
14. Gelatin Hydrolysis: positive
15. Nitrate Reduction: positive
I did gram stain it couple of times and its Gram negative rod. Not 100% sure since I'm not that confident.
And if you have links it would be highly appreciated.
Thanks in advance. God Bless.
Okay reading this thread has made me very worried.....we're scheduled to do our unknowns in a couple of weeks but we haven't done any of this stuff you are all posting about....we've only done negative stains, simple stains, and a gram stain (last week). I have no idea what citrate, indole, urease etc. is. why my teacher didn't teach us any of this I wonder?...
K nurse-one-day
693 Posts
yeah you have to be careful. not too long, not too short.