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microbiology culture

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aseptic technique can a pure culture be prepared directly from a mixed-broth or a mixed-slant culture

~Mi Vida Loca~RN, ASN, RN

Specializes in Emergency Dept. Trauma. Pediatrics. Has 6 years experience.

I know the answer to this, but it appears like you are simply posting test questions wanting others to answer them for you, if this is the case, no I won't answer it. You have to at least TRY or not make it so obvious. :stone

I know the answer to this, but it appears like you are simply posting test questions wanting others to answer them for you, if this is the case, no I won't answer it. You have to at least TRY or not make it so obvious. :stone

Ditto. I would be more than willing to help since I am graduating Sunday with my BS in Microbiology, but this seems fishy.

I have actually answered this in a short answer. I need an essay question. Aseptic Technique A broth culture cannot be presumed as a pure culture unless innoculated from an original pure culture and a mixed culture slant is very difficult to use to isolate colonies. A pure culture must be grown from an innoculation with cells from a single colony only. Therefore colonies that are very small or very close together introduce the risk of contamination during innocluation.

Thank you

Truly, there is nothing fishy about this. I am not sure what others are posting but I am not "others". I do apologize for both of you feeling that I may be doing whatever it is. The truth is I do have a BS in health care mgmt now working towards my pre reqs RN. This is an on line micro class which is extremely difficulty. I also have my another friend who is a PA trying to help me with the materials that have been provided for me. If you know of a micro web site where I can do further research then please send it to me. I am not asking for nothing else but guidance.

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