independent, dependent, and controlled variables for report? help

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I have a lab report due over anatomy physiology II and I am confused. Digestion of protein:

we used pepsin (Acidic) as the enzyme and the substrate albumin. Biuret was the reagent. Please help. Temperature also played a role with the digestion. what are the variables? when I went to the science tutors for lab they told me

"people make it more complicated than it really is. just put it in plain words."

That was all the assistance I got from the tutors. HELP

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Specializes in Critical Care.

The independent variable is the component that was manipulated in order to cause an observable change in another component (which is the dependent variable). In a well-designed experiment there is only one independent variable. Controlled variables remain the same throughout.

So for example, if you were testing how sunlight affects plant growth the independent variable would be the amount of sunlight the plant gets, the dependent variable would be the amount that the plant grew, and other factors like soil, water, plant food, etc. would all be controlled variables. Based on the experiment you performed in lab, what do you think the variables were?

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The independent variable is the component that was manipulated in order to cause an observable change in another component (which is the dependent variable). In a well-designed experiment there is only one independent variable. Controlled variables remain the same throughout.

So for example, if you were testing how sunlight affects plant growth the independent variable would be the amount of sunlight the plant gets, the dependent variable would be the amount that the plant grew, and other factors like soil, water, plant food, etc. would all be controlled variables. Based on the experiment you performed in lab, what do you think the variables were?

Would the independent variable be the temperature needed for the low ph of the pepsin to work and the dependent variable be the substrate albumin which was digested?

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Read your question carefully and it answers itself:

You placed biuret to albumin to see if would evoke any change. The pH and temperature had remain within specific levels in order for any change to take place.

Independent - biuret

Dependent - albumin

Constant - pH and Temp

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