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Discussion

Micro Unknown

Hi everyone! I need your help IDing my unknown.

I ran some tests and here are the results:

1. Starch Hydrolysis: positive

2. OF Glucose: negative

3. Fermentation Tube: Glucose: positive, gas negative. Lactose: negative, gas negative. Sucrose: positive, gas negative

4. Citrate: negative

5. Urease: positive, slow

6. Phenylalanine: negative

7. Hydrogen sulfide: negative

8. Motility: negative

9. Ornithine: negative

10. Indole: negative

11. Oxidase: negative. (Although I'm not that sure cause it only turned blue. not pink to blue to black.)

12. Catalase: posiive

13. MRVP: negative

14. Gelatin Hydrolysis: positive

15. Nitrate Reduction: positive

I did gram stain it couple of times and its Gram negative rod. Not 100% sure since I'm not that confident.

And if you have links it would be highly appreciated.

Thanks in advance. God Bless.

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    OP, I'm resurrecting this thread, because I'm wondering how did you do? I was just curious.

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    Okay reading this thread has made me very worried.....we're scheduled to do our unknowns in a couple of weeks but we haven't done any of this stuff you are all posting about....we've only done negative stains, simple stains, and a gram stain (last week). I have no idea what citrate, indole, urease etc. is. :barf02: why my teacher didn't teach us any of this I wonder?...

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