Don't get discourage. I don't think you've done anything wrong. Could it Aerotolerant anaerobe? They are microorganisms that can not use O2, but are no harmed by it either. So, there would be growth through out but not much, if any, at the top. I hope this helps. We are finish with our unknowns and cleaning up lab. So, I remember the gussing game hoping I could get some help from somewhere. Well, I never thought to check here. I've been so busy, this is the first time in awhile I have actually been on here. Well, I am saying all of that to say this even though I didn't have the help I still did very well on my paper. It was 13 pages long and I got a 95 on it. The best advice I can give you is to look at all of the test that would help you to narrow it down. Ex: Indole (SIMS), the Phenylalanine Deamination, Oxidase, EMB, Endo, etc. I hope I was able to help. If you have anymore questions post a reply. God Speed to You!! You will get it. PS, try to keep a good journal entry. What I mean by that is go ahead and explain exactly what the test shows. Not that it is just neg or pos. Go ahead and take the few extra minutes to write it in with the results. This way you would have a better understanding of it. Night! :) :balloons: