Testing the patient is not helpful - in fact it can be counterproductive If it is a high risk injury (ie large bore needle used to withdraw blood), it is worth staying on PEP even if they are negative because they could be in the window period (and especially infectious) If it is a low risk injury, management won't change if you know they are positive, but you will worry needlessly until you are confirmed negative at 3 months post-exposure. It sounds as though you are in the US. Note that the last time anyone got HIV at work in the US was in 2008 (And the last case before that was in 1999)