Generally, if someone overtly asks about benefits early in the interview it can be viewed negatively. The interview (particularly if it is with the hiring manager, not with HR) is the time to understand the unit's culture, leadership and ultimately determine if the job is a good fit for you. Usually, there isn't enough time to ask about benefits in that scenario. Astute questions about work-life balance, clinical ladders, coaching and development practices and how schedule requests are handled can help uncover information about benefits without asking overtly. By all means in a subsequent interview or when HR calls to offer a position, I agree with MRChicagoRN, have your list of questions developed and respectfully seek the answers to those questions prior to reaching your decision about the offer. Remember, this is your chance to interview this employer, just as much as it's the prospective employers chance to interview you.