Hamm

I was thinking I messed up the SIM but there was gas in the lactose so perhaps not. Thanks :)

Yeah they gave us Klebsiella to do other tests in the lab. The list they gave was the list of unknowns they used.

That's the problem, we only had two lab days to do our tests. One day to do them and the next week was viewing the results. It wasn't possible to do the SIM test again and by the time I figured out I had a problem, after I was doing my report, doing any other tests was not an option.

I am hoping someone here can help. I have a problem. Either I did my SIM wrong (likely) or my capsule stain wrong (pretty unlikely). What happened with the SIM was that I read the directions wrong. I stab inoculated it 3 times instead of once. I am not sure if I throw out the results completely or what. If I do, I know I have Citrobacter. If I don't, I would know I have Klebsiella. Only problem is that would mean my capsule stain was wrong because it was negative. I am not sure how I could have screwed that up. Here's what I have: Gram negative rod MacConkey neg or undetermined (colony was cream/slightly pink) Citrate positive SIM negative, all of it. Possibly screwed this up Deaminase negative Glucose positive +CO2 Lactose positive +CO2 Capsule stain negative (again, could have messed it up, not sure how) Possible unknowns: Serratia Streptococcus faecalis Shigella Salmonella Klebsiella Staphylococcus aureus Enterobacter Proteus vulgaris Citrobacter E. coli Staphylococcus epidermis Any insight appreciated