Protein synthesis question

[labchic]

Hi every body. I have a quick question on Protein synthesis. Ok....does it got from the non template strand of DNA to the Template strand of Dna. Then to the mRNA then to the tRna to amino acid? I know it's a pretty simple way of putting it but sometimes I've got to keep it simple. And is the anti codon just a stop codon? Any help would be appreciated!!

[Wolfee2010]

this came straight form my A&P notes:

Roles of the Three Types of RNA

Messenger RNA (mRNA) carries the genetic information from DNA in the nucleus to the ribosomes in the cytoplasm

Transfer RNAs (tRNAs) bound to amino acids base pair with the codons of mRNA at the ribosome to begin the process of protein synthesis

Ribosomal RNA (rRNA) is a structural component of ribosomes

Transcription

Transfer of information from the sense strand of DNA to RNA

Transcription factor

Loosens histones from DNA in the area to be transcribed

Binds to promoter, a DNA sequence specifying the start site of RNA synthesis

Mediates the binding of RNA polymerase to promoter

Transcription: RNA Polymerase

An enzyme that oversees the synthesis of RNA

Unwinds the DNA template

Adds complementary ribonucleoside triphosphates on the DNA template

Joins these RNA nucleotides together

Encodes a termination signal to stop transcription

Overview of Transcription (Fig. 3.35)

Initiation of Translation

A leader sequence on mRNA attaches to the small subunit of the ribosome

Methionine-charged initiator tRNA binds to the small subunit

The large ribosomal unit now binds to this complex forming a functional ribosome

Information Transfer from DNA to RNA

DNA triplets are transcribed into mRNA codons by RNA polymerase

Codons base pair with tRNA anticodons at the ribosomes

Amino acids are peptide bonded at the ribosomes to form polypeptide chains

Start and stop codons are used in initiating and ending translation

i hope that helps a little bit.. i know its wordy but i think it answers your question

[TheSquire, DNP, APRN, NP]

It doesn't "go" from the non-template to the template strand of DNA. Both are there the entire time. Helicase just unzips them, then the RNA Polymerase transcribes off the template strand to get the mRNA. Ribosomes (very important things, mind you, as they are ribozymes) latch onto the mRNA, chug along until they hit an AUG, then fetch a methionine tRNA whose anticodon matches up to the codon on the mRNA, and then ratchets along codon-by-codon til it hits a stop codon, when it releases the polypeptide that formed as the tRNAs got brought into the ribosome.

Make more sense now? You really shouldn't try to simplify transciption/translation, since what you're being taught is already very simplified as it is.