Micro Unknow Help Needed please

  1. I am struggling with unknown in microbiology class
    My unknown is E Coli
    if you have info about how to make flow chart or
    if you have sample of flow chart
    and how to write a report
    I would really appreciate that
    Please email me.
    I really need help ..
    Last edit by Nurse Ratched on Apr 23, '05 : Reason: removed email addy
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    Joined: Apr '05; Posts: 98; Likes: 6

    6 Comments

  3. by   tridil2000
    Quote from earthlover123
    I am struggling with unknown in microbiology class
    My unknown is gram positive cocci ... ( S. aureus )
    if you have info about how to make flow chart or
    if you have sample of flow chart
    and how to write a report
    I would really appreciate that
    I really need help ..
    google it.
    Last edit by Nurse Ratched on Apr 23, '05
  4. by   Indy
    For our microbiology class, I don't remember if it was a lab final or just a lab assignment, we had to figure out an unknown. However, the information for how to do this was in our book, in our lab book, and in a handout that the teacher gave us.

    In other words, in the lab manual there were many assignments on different types of tests. They started from general, like the gram stain to figure out if you've got a gram negative or gram positive organism. Then you did some more testing- like the 3 different tests that turn yellow with glucose metabolism, and so on. I can't remember them all. We had a lab that we did that was full of just practicing the tests, and then started on our unknowns. We got a test tube, numbered, (the teacher had a list of the numbers and what organism was supposed to be in the tube for her reference), and set up the required number of tests, labeling each tube so we'd know what test it was, and stuffed it in an incubator for 48 hours.

    Two days later we had to go over each test tube and write down our results in detail, and then use the material to figure out what organism it was. Now, the reason I'm saying all this is, that if you know what your unknown IS, then you should have done a battery of tests to figure it out. So, making a flowchart of what you did shouldn't be that difficult:

    Gram stain positive or Gram stain negative

    If positive, what test is next? If negative, what's next?

    ....with little boxes around the items, and lines between them. There should be examples in your microbiology book. But if you're creating a flowchart then it should be a record of what YOU did, not copied out of the book.

    This assignment is a lot of fun and it looks at lots of things: your understanding of the material, exactness of your method, ability to interpret results, and ability to report your results. Good luck with your flowchart!

    -Indy
  5. by   MryRose
    Quote from earthlover123
    I am struggling with unknown in microbiology class
    My unknown is E Coli
    if you have info about how to make flow chart or
    if you have sample of flow chart
    and how to write a report
    I would really appreciate that
    I really need help ..
    A flow chart is really simply a diagram of the steps that you took to determine what bacteria you have. How did you determine it is Escherichia Coli?

    Steps would include a gram staining, oxidase, indole, TSI etc... your flow chart should only include the tests that you actually do. Keep acurate and clear notes of each test you perform, the results of positive/negative, color changes, pH changes etc.

    If you are confused, contact your professor and they should be able to guide you. We just started the unkowns too and at first is was overwhelming trying to determine what tests to perform when. We have a great guide in our lab book to help us, your lab book probably has one too.

    I think it's a fun ending to this class.

    Hugs!

    MaryRose
    Last edit by Nurse Ratched on Apr 23, '05
  6. by   dmrnurse
    Click on the gram negative link at this site and you will see a flow chart for the tests that confirm E. coli. Are you sure that your unknown is E. coli? If so, this chart should help you create your document. For my micro class, we had to follow a flow chart to identify our unknowns. Good luck.

    http://www.kcom.edu/faculty/chamberl...idlab/gram.htm
  7. by   truern
    ??????

    You already know your unknown??
  8. by   earthlovers123
    Quote from dmrnurse
    Click on the gram negative link at this site and you will see a flow chart for the tests that confirm E. coli. Are you sure that your unknown is E. coli? If so, this chart should help you create your document. For my micro class, we had to follow a flow chart to identify our unknowns. Good luck.

    http://www.kcom.edu/faculty/chamberl...idlab/gram.htm
    WOW , Thank you

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