Absolutely!! When I worked in PACU, in the holding room, I had some standard jokes, like "OK, I know your jealous of my hat, so I have a bonnet for you too!" If one patient was still in PACU when I used the line on the new pt, I would look at them and wink, and they would always laugh.
Now in Home health, when I call to make a morning appt, and the person says, well, I won't even be dressed yet, I say, "That's OK, we don't take off any pints for you being in your pajamas." They always laugh.
I have a whole long list of these kinds of "standard" jokes to ease the tension. I don't always use them, in some cases it is inappropriate, but it sure helps when pt's have a good sense of humor too! Thankfully, I have never worked with picklepusses who can't appreciate a little humor. Good topic!